Typical Testing Data/Standard Curve (for reference only)
Rat Myc proto-oncogene protein ELISA Kit | Myc elisa kit
Rat Myc proto-oncogene protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myc proto-oncogene protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myc proto-oncogene protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myc proto-oncogene protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Myc proto-oncogene protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Rat mRNA also has a similarly placed CUG upstream of the AUG start site, suggesting that it may also produce two Myc proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.
Protein type: Oncoprotein; Transcription factor; DNA-binding; Nucleolus
Cellular Component: axon; cytoplasm; mitochondrion; nuclear body; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex; spindle
Molecular Function: DNA binding; double-stranded DNA binding; protein binding; protein complex binding; protein dimerization activity; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding
Biological Process: amino acid transport; B cell apoptosis; caspase activation; cell cycle arrest; cell proliferation; cellular iron ion homeostasis; cellular response to insulin stimulus; chromatin remodeling; chromosome organization and biogenesis; detection of mechanical stimulus involved in sensory perception of sound; DNA damage response, signal transduction resulting in induction of apoptosis; flavonoid metabolic process; G0 to G1 transition; glucose metabolic process; hypothalamus development; in utero embryonic development; inner mitochondrial membrane organization and biogenesis; lactic acid secretion; MAPKKK cascade; middle ear morphogenesis; negative regulation of cell division; negative regulation of fibroblast proliferation; negative regulation of glucose import; negative regulation of monocyte differentiation; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; ovarian follicle development; ovulation; pathogenesis; pigmentation; positive regulation of B cell apoptosis; positive regulation of caspase activity; positive regulation of catalytic activity; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of glycolysis; positive regulation of mesenchymal cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein processing; pyruvate transport; re-entry into mitotic cell cycle; regulation of apoptosis; regulation of gene expression; regulation of mitotic cell cycle; regulation of oxidative phosphorylation; regulation of telomere maintenance; regulation of transcription, DNA-dependent; response to alkaloid; response to DNA damage stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to gamma radiation; response to radiation; response to wounding; skeletal morphogenesis; transcription from RNA polymerase II promoter; transcription initiation; transcription, DNA-dependent; transformation of host cell by virus; ureteric bud branching; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Research Articles on Myc
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Product Notes
The Rat Myc myc (Catalog #AAA289810) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA289810 ELISA Kit recognizes Rat Myc. It is sometimes possible for the material contained within the vial of "Myc proto-oncogene protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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