Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Muscarinic acetylcholine receptor M2 (CHRM2) Standard Curve.)
Rat Muscarinic Acetylcholine Receptor M2 (CHRM2) ELISA Kit | CHRM2 elisa kit
Rat Muscarinic Acetylcholine Receptor M2 (CHRM2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Muscarinic acetylcholine receptor M2 (CHRM2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Muscarinic acetylcholine receptor M2 (CHRM2) Standard Curve.)
Background: He is a muscarinic acetylcholine receptor.M2 muscarinic receptors act via a Gi type receptor, which causes a decrease in cAMP in the cell, generally leading to inhibitory-type effects.In addition, they modulate muscarinic potassium channels. In the heart, this contributes to a decreased heart rate.The M2 muscarinic receptors are located in the heart, where they act to slow the heart rate down to normal sinus rhythm after stimulatory actions of the sympathetic nervous system, by slowing the speed of depolarization. They also reduce contractile forces of the atrial cardiac muscle, and reduce conduction velocity of the atrioventricular node (AV node). However, they have no effect on the contractile forces of the ventricular muscle.
NCBI and Uniprot Product Information
NCBI Description
may inhibit cholinergic-mediated bronchoconstriction; may regulate bladder contraction [RGD, Feb 2006]
Uniprot Description
mAChR M2: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD). MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Cellular Component: asymmetric synapse; postsynaptic membrane; cell soma; membrane; integral to plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction
Molecular Function: drug binding; G-protein coupled acetylcholine receptor activity
Biological Process: regulation of smooth muscle contraction; synaptic transmission; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); acetylcholine receptor signaling, muscarinic pathway; response to virus; regulation of heart contraction
Research Articles on CHRM2
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Product Notes
The Rat CHRM2 chrm2 (Catalog #AAA9712590) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712590 ELISA Kit recognizes Rat CHRM2. It is sometimes possible for the material contained within the vial of "Muscarinic Acetylcholine Receptor M2 (CHRM2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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