Rabbit CHRM2 Polyclonal Antibody | anti-CHRM2 antibody

CHRM2 Antibody

Cat. Num. AAA9413727

• Gene Names: CHRM2; HM2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: CHRM2; Polyclonal Antibody; CHRM2 Antibody; FLJ43243; HM2; MGC120006; MGC120007; anti-CHRM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total CHRM2 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 466

• Applicable Applications for anti-CHRM2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human CHRM2.
• Target Name: CHRM2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of 293 cell line, using CHRM2 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using CHRM2 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-CHRM2 antibody

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.

Product Categories/Family for anti-CHRM2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502817
• NCBI GeneID: 1129
• NCBI Accession #: NP_000730.1
• NCBI GenBank Nucleotide #: NM_000739.2
• UniProt Accession #: P08172; Q4VBK6; Q9P1X9
• Molecular Weight: 51,715 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor M2
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 2
• NCBI Official Symbol: CHRM2
• NCBI Official Synonym Symbols: HM2
• NCBI Protein Information: muscarinic acetylcholine receptor M2
• UniProt Protein Name: Muscarinic acetylcholine receptor M2
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM2
• UniProt Entry Name: ACM2_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR M2: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD). MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 7q31-q35

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: G-protein coupled acetylcholine receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; G-protein coupled receptor protein signaling pathway; nervous system development; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); acetylcholine receptor signaling, muscarinic pathway; response to virus; muscarinic acetylcholine receptor, phospholipase C activating pathway; regulation of heart contraction