Rat Motilin (MTL) ELISA Kit | MTL elisa kit

Rat Motilin (MTL) ELISA Kit

Cat. Num. AAA2023132

• Reactivity: Rat
• Synonyms: Motilin (MTL); Rat Motilin (MTL) ELISA Kit; MLN; MAP; Promotilin; Motilin-associated peptide; MTL elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Motilin (MTL).; No significant cross-reactivity or interference between Motilin (MTL) and analogues was observed.
• Sequence Length: 114

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 74.07-6000pg/mL
• Sensitivity: < 29.51pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Motilin (MTL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Motilin (MTL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTL elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Motilin (MTL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Motilin (MTL) and unlabeled Motilin (MTL) (Standards or samples) with the pre-coated antibody specific to Motilin (MTL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Motilin (MTL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Motilin (MTL) in the sample.

Product Categories/Family for MTL elisa kit

Endocrinology; Gastroenterology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 386955
• NCBI GeneID: 4295
• UniProt Accession #: P12872; Q2M1L2; Q5T975; Q6NSY7; B7ZLR7; E9PDN2; J3KN51
• Molecular Weight: 12,849 Da
• NCBI Official Full Name: motilin
• NCBI Official Synonym Full Names: motilin
• NCBI Official Symbol: MLN
• NCBI Protein Information: promotilin
• UniProt Protein Name: Promotilin
• UniProt Gene Name: MLN
• UniProt Synonym Gene Names: MAP
• UniProt Entry Name: MOTI_HUMAN

NCBI Description

This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

MLN: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Belongs to the motilin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling