Rat Monokine Induced By Interferon Gamma (MIg) ELISA Kit | MIg elisa kit

Rat Monokine Induced By Interferon Gamma (MIg) ELISA Kit

Cat. Num. AAA2703909

• Reactivity: Rat
• Synonyms: Monokine Induced By Interferon Gamma (MIg); Rat Monokine Induced By Interferon Gamma (MIg) ELISA Kit; CXCL9; CMK; Humig; MI-G; SCYB9; Crg-10; Chemokine (C-X-C motif) ligand 9; Small-inducible cytokine B9; Gamma-interferon-induced monokine; MIg elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of MIg. No significant cross-reactivity or interference between MIg and analogues was observed.
• Sequence Length: 125

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: < 13.1pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MIg were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MIg were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIg elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MIg in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MIg. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MIg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MIg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MIg in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 161579144
• NCBI GeneID: 513990
• UniProt Accession #: A9QWP9
• Molecular Weight: 14,106 Da
• NCBI Official Full Name: monokine induced by interferon gamma
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 9
• NCBI Official Symbol: CXCL9
• NCBI Protein Information: C-X-C motif chemokine 9
• UniProt Protein Name: C-X-C motif chemokine 9
• UniProt Gene Name: CXCL9

Uniprot Description

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3 ().