Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Matrix metallopeptidase 8 (MMP8) Standard Curve.)
Rat Matrix Metallopeptidase 8 (MMP8) ELISA Kit | MMP8 elisa kit
Rat Matrix Metallopeptidase 8 (MMP8) ELISA Kit
Reactivity
Rat
Synonyms
Matrix Metallopeptidase 8 (MMP8); Rat Matrix Metallopeptidase 8 (MMP8) ELISA Kit; MMP8; CLG1; HNC; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase); MMP8 elisa kit
Reactivity
Rat
Specificity
Rat Matrix metallopeptidase 8 (MMP8) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MMP8. No significant cross-reactivity or interference between Rat MMP8 and analogues was observed.
Sequence Length
466
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Matrix metallopeptidase 8 (MMP8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Matrix metallopeptidase 8 (MMP8) Standard Curve.)
Related Product Information for MMP8 elisa kit
Description: This Rat Matrix metallopeptidase 8 (MMP8) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP8 in samples. An antibody specific for MMP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MMP8 degrades fibrillar collagens types I, II, III, aggrecan, serpins and alpha 2 macroglobulin. The substrate specificity of collagenases is variable: MMP1 degrades type III collagen more efficiently than type I or type II collagen, whereas MMP8 is more potent in degrading type I collagen than type III or type II collagen. Unlike other members, MMP8 is expressed exclusively in inflammatory conditions. MMP8 is highly expressed in the postpartum uterus, and it is thought to be involved in the postpartum involution of the uterus. MMP8 is also the predominant collagenase expressed in ulcers and healing wounds. MMP8 is very similar to MMP1, sharing 57 % amino acid identity. MMP8 is heavily glycosylated, and the zymogen has a mass of 85 Kd.
Background: MMP8 degrades fibrillar collagens types I, II, III, aggrecan, serpins and alpha 2 macroglobulin. The substrate specificity of collagenases is variable: MMP1 degrades type III collagen more efficiently than type I or type II collagen, whereas MMP8 is more potent in degrading type I collagen than type III or type II collagen. Unlike other members, MMP8 is expressed exclusively in inflammatory conditions. MMP8 is highly expressed in the postpartum uterus, and it is thought to be involved in the postpartum involution of the uterus. MMP8 is also the predominant collagenase expressed in ulcers and healing wounds. MMP8 is very similar to MMP1, sharing 57 % amino acid identity. MMP8 is heavily glycosylated, and the zymogen has a mass of 85 Kd.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neutrophil collagenase
NCBI Official Synonym Full Names
matrix metallopeptidase 8
NCBI Official Symbol
Mmp8
NCBI Protein Information
neutrophil collagenase
UniProt Protein Name
Neutrophil collagenase
Protein Family
UniProt Gene Name
Mmp8
UniProt Synonym Gene Names
MMP-8
UniProt Entry Name
MMP8_RAT
NCBI Description
mouse homolog is a neutral metalloproteinase of the fibrillar collagenase family [RGD, Feb 2006]
Uniprot Description
Can degrade fibrillar type I, II, and III collagens.
Research Articles on MMP8
Similar Products
Product Notes
The Rat MMP8 mmp8 (Catalog #AAA9712687) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712687 ELISA Kit recognizes Rat MMP8. It is sometimes possible for the material contained within the vial of "Matrix Metallopeptidase 8 (MMP8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
