Rat mast cell tryptase (MCT) ELISA Kit | MCT elisa kit

Rat mast cell tryptase (MCT) ELISA Kit

Cat. Num. AAA265220

• Reactivity: Rat
• Synonyms: mast cell tryptase (MCT); Rat mast cell tryptase (MCT) ELISA Kit; MCT elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 274

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: Up to 0.5 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCT elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat MCT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 556556
• Molecular Weight: 30,508 Da
• NCBI Official Full Name: mast cell tryptase
• UniProt Protein Name: Tryptase beta-2
• Protein Family: 2-methylfumaryl-CoA isomerase
• UniProt Gene Name: Tpsb2
• UniProt Synonym Gene Names: Mcp6; Mcpt6; Tryptase-2; rMCP-6
• UniProt Entry Name: TRYB2_RAT

NCBI Description

serine protease; plays a role during the inflammatory process [RGD, Feb 2006]

Uniprot Description

TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily.

Protein type: EC 3.4.21.59; Secreted, signal peptide; Protease; Secreted

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: peptidase activity; heparin binding; serine-type endopeptidase activity

Biological Process: proteolysis