Typical Testing Data/Standard Curve (for reference only)
Rat Macrophage Stimulating Protein (MSP) ELISA Kit | MSP elisa kit
Rat Macrophage Stimulating Protein (MSP) ELISA Kit
Gene Names
MST1; MSP; HGFL; NF15S2; D3F15S2; DNF15S2
Reactivity
Rat
Synonyms
Macrophage Stimulating Protein (MSP); Rat Macrophage Stimulating Protein (MSP) ELISA Kit; MST1; HGFL; NF15S2; Hepatocyte Growth Factor-Like Protein; Macrophage Stimulating 1; MSP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Macrophage Stimulating Protein (MSP).
No significant cross-reactivity or interference between Macrophage Stimulating Protein (MSP) and analogues was observed.
No significant cross-reactivity or interference between Macrophage Stimulating Protein (MSP) and analogues was observed.
Sequence Length
711
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Macrophage Stimulating Protein (MSP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Macrophage Stimulating Protein (MSP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MSP elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Stimulating Protein (MSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Stimulating Protein (MSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Stimulating Protein (MSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Stimulating Protein (MSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MSP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
80,320 Da
NCBI Official Full Name
macrophage-stimulating protein
NCBI Official Synonym Full Names
macrophage stimulating 1
NCBI Official Symbol
MST1
NCBI Official Synonym Symbols
MSP; HGFL; NF15S2; D3F15S2; DNF15S2
NCBI Protein Information
hepatocyte growth factor-like protein
UniProt Protein Name
Hepatocyte growth factor-like protein
Protein Family
UniProt Gene Name
MST1
UniProt Synonym Gene Names
D3F15S2; DNF15S2; HGFL; MSP
UniProt Entry Name
HGFL_HUMAN
NCBI Description
The protein encoded by this gene contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. [provided by RefSeq, Jan 2010]
Uniprot Description
MSP: Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. Belongs to the peptidase S1 family. Plasminogen subfamily.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p21
Molecular Function: serine-type endopeptidase activity
Biological Process: sperm motility; spermatogenesis; proteolysis; embryo implantation
Research Articles on MSP
Similar Products
Product Notes
The Rat MSP mst1 (Catalog #AAA2024253) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024253 ELISA Kit recognizes Rat MSP. It is sometimes possible for the material contained within the vial of "Macrophage Stimulating Protein (MSP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
