Typical Testing Data/Standard Curve (for reference only)
Macrophage Migration Inhibitory Factor (MIF) ELISA Kit | MIF elisa kit
Rat Macrophage Migration Inhibitory Factor (MIF) Wide-range ELISA Kit
No significant cross-reactivity or interference between Wide-range Macrophage Migration Inhibitory Factor (MIF) and analogues was observed.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Macrophage Migration Inhibitory Factor (MIF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI Description
inhibits random migration of macrophages and is involved in the pathogenesis of several inflammatory diseases [RGD, Feb 2006]
Uniprot Description
MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.
Protein type: Amino Acid Metabolism - tyrosine; EC 5.3.2.1; Isomerase; Apoptosis; Amino Acid Metabolism - phenylalanine; EC 5.3.3.12; Cytokine; Cell development/differentiation
Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; intracellular; nucleus; myelin sheath; vesicle
Molecular Function: phenylpyruvate tautomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; protein complex binding; cytokine activity; dopachrome isomerase activity; chemoattractant activity; receptor binding
Biological Process: skin development; positive regulation of immune response; wound healing; DNA damage response, signal transduction by p53 class mediator; metabolic process; response to menaquinone; positive regulation of potassium ion transport; response to glucocorticoid stimulus; drinking behavior; response to hormone stimulus; positive regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of cellular protein metabolic process; response to lipopolysaccharide; positive regulation of smooth muscle cell migration; prostaglandin biosynthetic process; response to estradiol stimulus; response to insulin stimulus; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of glucose import; positive regulation of MAP kinase activity; negative regulation of myeloid cell apoptosis; response to vitamin E; positive regulation of fibroblast proliferation; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; hair follicle development; positive regulation of acute inflammatory response; positive regulation of B cell proliferation; inflammatory response; aging; response to drug; positive regulation of axon regeneration; response to inorganic substance; carboxylic acid metabolic process; cell aging; positive regulation of peptidyl-serine phosphorylation; brain renin-angiotensin system; regulation of cell proliferation; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; response to heat; response to mechanical stimulus; positive regulation of glycolysis; innate immune response; spermatogenesis; brain development; positive regulation of protein amino acid phosphorylation; response to progesterone stimulus; positive regulation of phosphorylation; positive regulation of cytokine secretion; negative regulation of apoptosis
Research Articles on MIF
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Product Notes
The Rat MIF mif (Catalog #AAA2709282) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709282 ELISA Kit recognizes Rat MIF. AAA Biotech's Macrophage Migration Inhibitory Factor (MIF) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the MIF mif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophage Migration Inhibitory Factor (MIF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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