Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Asc-type amino acid transporter 1 (SLC7A10) Standard Curve.)
Human Asc-Type Amino Acid Transporter 1 (SLC7A10) ELISA Kit | SLC7A10 elisa kit
Human Asc-Type Amino Acid Transporter 1 (SLC7A10) ELISA Kit
Gene Names
SLC7A10; ASC1; asc-1; HASC-1
Reactivity
Human
Synonyms
Asc-Type Amino Acid Transporter 1 (SLC7A10); Human Asc-Type Amino Acid Transporter 1 (SLC7A10) ELISA Kit; SLC7A10; FLJ20839; HASC-1; asc-1; asc-type amino acid transporter 1; solute carrier family 7; (cationic amino acid transporter; y+ system) member 10; member 10; SLC7A10 elisa kit
Reactivity
Human
Specificity
Human Asc-type amino acid transporter 1 (SLC7A10) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC7A10. No significant cross-reactivity or interference between Human SLC7A10 and analogues was observed.
Sequence Length
523
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Asc-type amino acid transporter 1 (SLC7A10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Asc-type amino acid transporter 1 (SLC7A10) Standard Curve.)
Related Product Information for SLC7A10 elisa kit
Description: This Human Asc-type amino acid transporter 1 (SLC7A10) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A10 in samples. An antibody specific for SLC7A10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC7A10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SLC7A10, in association with 4F2HC (SLC3A2), mediates high-affinity transport of D-serine and several other neutral amino acids.The deduced 530-amino acid protein contains 12 transmembrane regions and a conserved cysteine residue (cys160) in the putative extracellular loop between transmembrane domains 3 and 4. This residue was presumed to be linked to 4f2hc via a disulfide bond. Northern blot analysis detected a 1.9-kb transcript in mouse brain and lung, with weaker expression in placenta. A single 4.4-kb transcript was expressed in small intestine.ASC1 shares 91% identity with mouse Asc1. Northern blot analysis detected 1.3-, 2.0-, 4.3-, and 6.0-kb transcripts in brain, heart, placenta, skeletal muscle, and kidney. The 2.0-kb transcript predominated in brain.
Background: SLC7A10, in association with 4F2HC (SLC3A2), mediates high-affinity transport of D-serine and several other neutral amino acids.The deduced 530-amino acid protein contains 12 transmembrane regions and a conserved cysteine residue (cys160) in the putative extracellular loop between transmembrane domains 3 and 4. This residue was presumed to be linked to 4f2hc via a disulfide bond. Northern blot analysis detected a 1.9-kb transcript in mouse brain and lung, with weaker expression in placenta. A single 4.4-kb transcript was expressed in small intestine.ASC1 shares 91% identity with mouse Asc1. Northern blot analysis detected 1.3-, 2.0-, 4.3-, and 6.0-kb transcripts in brain, heart, placenta, skeletal muscle, and kidney. The 2.0-kb transcript predominated in brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
asc-type amino acid transporter 1
NCBI Official Synonym Full Names
solute carrier family 7 member 10
NCBI Official Symbol
SLC7A10
NCBI Official Synonym Symbols
ASC1; asc-1; HASC-1
NCBI Protein Information
asc-type amino acid transporter 1
UniProt Protein Name
Asc-type amino acid transporter 1
Protein Family
UniProt Gene Name
SLC7A10
UniProt Synonym Gene Names
ASC1; Asc-1
NCBI Description
SLC7A10, in association with 4F2HC (SLC3A2; MIM 158070), mediates high-affinity transport of D-serine and several other neutral amino acids (Nakauchi et al., 2000 [PubMed 10863037]).[supplied by OMIM, Mar 2008]
Uniprot Description
Sodium-independent, high affinity transport of small neutral D- and L-amino acids. May play a role in the modulation of glutamatergic transmission through mobilization of D-serine at the glutamatergic synapse.
Research Articles on SLC7A10
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Product Notes
The Human SLC7A10 slc7a10 (Catalog #AAA9714254) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714254 ELISA Kit recognizes Human SLC7A10. It is sometimes possible for the material contained within the vial of "Asc-Type Amino Acid Transporter 1 (SLC7A10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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