Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Long-chain fatty acid transport protein 1 (SLC27A1) Standard Curve.)
Rat Long-Chain Fatty Acid Transport Protein 1 (SLC27A1) ELISA Kit | SLC27A1 elisa kit
Rat Long-Chain Fatty Acid Transport Protein 1 (SLC27A1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Long-chain fatty acid transport protein 1 (SLC27A1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Long-chain fatty acid transport protein 1 (SLC27A1) Standard Curve.)
Background: Transfected cells were grown in the presence of a fluorescent fatty acid analog and those that became positive for the dye were sorted and the cDNA recovered. Two classes of clones were identified: one encodes a 699-amino acid protein resembling rat liver fatty acyl CoA synthetase (FACL2), and the second encodes a 646-amino acid protein with 6 potential transmembrane domains. Northern blot analysis showed high levels of FATP in 3T3-L1 adipocytes, with a major 2.9-kb transcript and a minor 3.8-kb form. Expression increased 5-fold during differentiation of preadipocytes to adipocytes. In normal mouse tissues, FATP mRNA levels were highest in skeletal muscle, heart, and fat. Expression studies showed that FATP increased the uptake of labeled oleic acid by 3- to 4-fold, and similar effects were observed with other LCFAs.
NCBI and Uniprot Product Information
NCBI Description
involved in myocardial fatty acid uptake [RGD, Feb 2006]
Uniprot Description
SLC27A1: Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. The LFCA import appears to be hormone-regulated in a tissue-specific manner. In adipocytes, but not myocytes, insulin induces a rapid translocation of Fatp1 from intracellular compartments to the plasma membrane, paralleled by increased LFCA uptake. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LFCA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (By similarity)
Protein type: Ligase; Membrane protein, integral; Vesicle; EC 6.2.1.-; Lipid-binding; Mitochondrial
Cellular Component: caveola; endomembrane system; endoplasmic reticulum; integral to membrane; intracellular membrane-bound organelle; membrane; mitochondrial inner membrane; mitochondrion; plasma membrane
Molecular Function: fatty acid transporter activity; long-chain-fatty-acid-CoA ligase activity; nucleotide binding; protein binding; protein homodimerization activity; very-long-chain-fatty-acid-CoA ligase activity
Biological Process: adiponectin-mediated signaling pathway; cardiolipin biosynthetic process; fatty acid transport; long-chain fatty acid metabolic process; long-chain fatty acid transport; medium-chain fatty acid transport; phosphatidic acid biosynthetic process; phosphatidylcholine biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylglycerol biosynthetic process; phosphatidylinositol biosynthetic process; phosphatidylserine biosynthetic process; positive regulation of heat generation; response to cold; response to insulin stimulus
Research Articles on SLC27A1
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Product Notes
The Rat SLC27A1 slc27a1 (Catalog #AAA9711872) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711872 ELISA Kit recognizes Rat SLC27A1. It is sometimes possible for the material contained within the vial of "Long-Chain Fatty Acid Transport Protein 1 (SLC27A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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