Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Leukemia inhibitory factor (LIF) Standard Curve.)
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit | LIF elisa kit
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
Reactivity
Rat
Synonyms
Leukemia Inhibitory Factor (LIF); Rat Leukemia Inhibitory Factor (LIF) ELISA Kit; LIF; CDF; DIA; HILDA; D factor; cholinergic differentiation factor; differentiation inhibitory activity; differentiation stimulating factor; LIF elisa kit
Reactivity
Rat
Specificity
Rat Leukemia inhibitory factor (LIF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LIF. No significant cross-reactivity or interference between Rat LIF and analogues was observed.
Sequence Length
202
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Leukemia inhibitory factor (LIF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Leukemia inhibitory factor (LIF) Standard Curve.)
Related Product Information for LIF elisa kit
Description: This Rat Leukemia inhibitory factor (LIF) ELISA Kit employs a two-site sandwich ELISA to quantitate LIF in samples. An antibody specific for LIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Leukemia inhibitory factor, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukaemic cells. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF is normally expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. As embryonic stem cells are derived from the inner cell mass at the blastocyst stage, removing them from the inner cell mass also removes their source of LIF.
Background: Leukemia inhibitory factor, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukaemic cells. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF is normally expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. As embryonic stem cells are derived from the inner cell mass at the blastocyst stage, removing them from the inner cell mass also removes their source of LIF.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Leukemia inhibitory factor
NCBI Official Synonym Full Names
LIF, interleukin 6 family cytokine
NCBI Official Symbol
Lif
NCBI Protein Information
leukemia inhibitory factor
UniProt Protein Name
Leukemia inhibitory factor
Protein Family
UniProt Gene Name
Lif
UniProt Synonym Gene Names
LIF
UniProt Entry Name
LIF_RAT
NCBI Description
a secreted cytokine; involved in embryonic stem cell and myeloid cell growth and differentiation and stimulation of bone remodeling [RGD, Feb 2006]
Uniprot Description
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Research Articles on LIF
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Product Notes
The Rat LIF lif (Catalog #AAA9712362) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712362 ELISA Kit recognizes Rat LIF. It is sometimes possible for the material contained within the vial of "Leukemia Inhibitory Factor (LIF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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