Rat Large neutral amino acids transporter small subunit 2 (SLC7A8) ELISA Kit | SLC7A8 elisa kit

Rat Large neutral amino acids transporter small subunit 2 (SLC7A8) ELISA Kit

Cat. Num. AAA287460

• Gene Names: SLC7A8; 4F2LC-5
• Reactivity: Rat
• Synonyms: Large neutral amino acids transporter small subunit 2 (SLC7A8); Rat Large neutral amino acids transporter small subunit 2 (SLC7A8) ELISA Kit; LAT2; LPI-PC1; integral membrane protein E16H; SLC7A8 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat SLC7A8. No significant cross-reactivity or interference between Rat SLC7A8 and analogues was observed.
• Sequence Length: 535

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for SLC7A8 elisa kit

This assay employs a two-site sandwich ELISA to quantitate SLC7A8 in samples. An antibody specific for SLC7A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC7A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 130502150
• NCBI GeneID: 100009408
• NCBI Accession #: NP_001076151.1
• NCBI GenBank Nucleotide #: NM_001082682.1
• UniProt Accession #: Q9N1Q4
• Molecular Weight: 58,321 Da
• NCBI Official Full Name: large neutral amino acids transporter small subunit 2
• NCBI Official Symbol: SLC7A8
• NCBI Official Synonym Symbols: 4F2LC-5
• NCBI Protein Information: large neutral amino acids transporter small subunit 2
• UniProt Protein Name: Large neutral amino acids transporter small subunit 2
• Protein Family: Large neutral amino acids transporter
• UniProt Gene Name: SLC7A8

Uniprot Description

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.