Rat Lactoferrin (LTF) ELISA Kit | LTF elisa kit

Rat Lactoferrin (LTF) ELISA Kit

Cat. Num. AAA2020194

• Gene Names: LTF; LF; HLF2; GIG12; HEL110
• Reactivity: Rat
• Synonyms: Lactoferrin (LTF); Rat Lactoferrin (LTF) ELISA Kit; HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1; LTF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lactoferrin (LTF).; No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.
• Sequence Length: 475

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: < 2.84ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactoferrin (LTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactoferrin (LTF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LTF elisa kit

Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 2104522
• NCBI GeneID: 4057
• UniProt Accession #: P02788; O00756; Q16780; Q16785; Q16786; Q16789; Q5DSM0; Q8IU92; A8K9U8; B2MV13; B7Z4X2; E7EQH5
• Molecular Weight: 73,161 Da
• NCBI Official Full Name: lactoferrin, partial
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12; HEL110
• NCBI Protein Information: lactotransferrin
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN

NCBI Description

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; serine-type endopeptidase activity; iron ion binding

Biological Process: interaction with host; negative regulation of lipopolysaccharide-mediated signaling pathway; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; retinal homeostasis; positive regulation of osteoblast differentiation; innate immune response in mucosa; negative regulation of viral genome replication; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of viral reproduction; negative regulation of apoptosis