Human Lactase (LCT) RTU ELISA Kit | LCT rtu elisa kit

Human Lactase (LCT) ELISA Kit

Cat. Num. AAA4503560

• Gene Names: LCT; LAC; LPH; LPH1
• Reactivity: Human
• Synonyms: Lactase (LCT); Human Lactase (LCT) ELISA Kit; LAC; LPH; LPH1; Lactose-Phlorizin Hydrolase; LCT rtu elisa kit

• Reactivity: Human
• Sequence Length: 1927

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.05ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCT rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LCT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LCT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LCT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32481206
• NCBI GeneID: 3938
• NCBI Accession #: NP_002290.2
• NCBI GenBank Nucleotide #: NM_002299.3
• UniProt Accession #: P09848; Q4ZG58
• Molecular Weight: 218,587 Da
• NCBI Official Full Name: lactase-phlorizin hydrolase preproprotein
• NCBI Official Synonym Full Names: lactase
• NCBI Official Symbol: LCT
• NCBI Official Synonym Symbols: LAC; LPH; LPH1
• NCBI Protein Information: lactase-phlorizin hydrolase
• UniProt Protein Name: Lactase-phlorizin hydrolase
• Protein Family: Lactase-phlorizin hydrolase
• UniProt Gene Name: LCT
• UniProt Synonym Gene Names: LPH

NCBI Description

The protein encoded by this gene belongs to the glycosyl hydrolase 1 family of proteins. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme is integral to the plasma membrane and has both phlorizin hydrolase activity and lactase activity. Mutations in this gene are associated with congenital lactase deficiency. Polymorphisms in this gene are associated with lactase persistence, in which intestinal lactase activity persists at childhood levels into adulthood. [provided by RefSeq, Jan 2016]

Uniprot Description

LPH splits lactose in the small intestine.