Typical Testing Data/Standard Curve (for reference only)
Rat Interleukin-12 receptor subunit beta-2 (IL12RB2) ELISA Kit | IL12RB2 elisa kit
Rat Interleukin-12 receptor subunit beta-2 (IL12RB2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IL-12RB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-12RB2 antibody and an IL-12RB2-HRP conjugate. The assay sample and buffer are incubated together with IL-12RB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-12RB2 concentration since IL-12RB2 from samples and IL-12RB2-HRP conjugate compete for the anti-IL-12RB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-12RB2 from the sample, fewer sites are left to bind IL-12RB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-12RB2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
IL12RB2: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell cycle regulation; Receptor, cytokine
Chromosomal Location of Human Ortholog: 1p31.3-p31.2
Cellular Component: integral to plasma membrane; external side of plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein kinase binding
Biological Process: positive regulation of interferon-gamma production; peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; interferon-gamma production; response to lipopolysaccharide
Research Articles on IL12RB2
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Product Notes
The Rat IL12RB2 il12rb2 (Catalog #AAA7202973) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7202973 ELISA Kit recognizes Rat IL12RB2. It is sometimes possible for the material contained within the vial of "Interleukin-12 receptor subunit beta-2 (IL12RB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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