Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Rat Insulin ELISA Kit | INS elisa kit

Insulin: Rat, Ultra sensitive (ELISA)

Gene Names
INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
Reactivity
Rat
Synonyms
Insulin; Insulin: Rat; Ultra sensitive (ELISA); Ultra sensitive rat insulin; INS elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
110
Samples
Rat serum, plasma and fluid
Sensitivity
0.025 ng/ml
Intra-assay Precision
C.V. < 10%.
Inter-assay Precision
C.V. <10%.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for INS elisa kit
Principle of the Assay: This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and coincubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
References
1. Molecular mechanisms of insulin resistance in diabetes. Soumaya K. Adv Exp Med Biol. 2012;771:240-51.
2. The links between insulin resistance, diabetes, and cancer. Orgel E, Mittelman SD. Curr Diab Rep. 2013 13(2):213-22

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
insulin
NCBI Official Synonym Full Names
insulin
NCBI Official Symbol
INS
NCBI Official Synonym Symbols
IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
NCBI Protein Information
insulin
Protein Family

NCBI Description

This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq, May 2020]

Research Articles on INS

Similar Products

Product Notes

The Rat INS (Catalog #AAA398070) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA398070 ELISA Kit recognizes Rat INS. It is sometimes possible for the material contained within the vial of "Insulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.