Typical Testing Data/Standard Curve (for reference only)
Rat IGFBP-2 ELISA Kit | IGFBP-2 elisa kit
Rat IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IGFBP-2. You can calculate the concentration of Rat IGFBP-2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle
Molecular Function: protein binding; insulin-like growth factor I binding; insulin-like growth factor II binding
Biological Process: response to drug; regulation of insulin-like growth factor receptor signaling pathway; response to retinoic acid; response to lithium ion; response to glucocorticoid stimulus; female pregnancy; signal transduction; positive regulation of activated T cell proliferation; response to estradiol stimulus; cellular response to hormone stimulus; cellular protein metabolic process; response to mechanical stimulus; regulation of cell growth; response to nutrient; aging
Research Articles on IGFBP-2
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Product Notes
The Rat IGFBP-2 igfbp2 (Catalog #AAA2508647) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2508647 ELISA Kit recognizes Rat IGFBP-2. It is sometimes possible for the material contained within the vial of "IGFBP-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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