Rat High Mobility Group Protein B1 (HMGB-1) ELISA Kit | HMGB-1 elisa kit

Rat High Mobility Group Protein B1 (HMGB-1) ELISA Kit

Cat. Num. AAA3807918

• Gene Names: HMGB1; HMG1; HMG3; HMG-1; SBP-1
• Reactivity: Rat
• Synonyms: High Mobility Group Protein B1 (HMGB-1); Rat High Mobility Group Protein B1 (HMGB-1) ELISA Kit; HMGB-1 elisa kit

• Reactivity: Rat
• Sequence Length: 215

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 7.5ng/ml-120ng/ml
• Sensitivity: 0.1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HMGB-1 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHMGB-1 in the sample, This HMGB-1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHMGB-1 concentration. The concentration ofHMGB-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504425
• NCBI GeneID: 3146
• NCBI GenBank Nucleotide #: NP_002119.1
• UniProt Accession #: P09429
• NCBI Official Full Name: high mobility group protein B1 isoform 1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1
• UniProt Entry Name: HMGB1_HUMAN

NCBI Description

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus

Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; transcription factor activity; DNA bending activity; transcription factor binding; single-stranded DNA binding; chemoattractant activity

Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; innate immune response; DNA ligation during DNA repair; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding