Rat HMGB-1 (High Mobility Group Protein B1) CLIA Kit | HMGB-1 clia kit

Rat HMGB-1 (High Mobility Group Protein B1) CLIA Kit

Cat. Num. AAA2530897

• Gene Names: HMGB1; HMG1; HMG3; HMG-1; SBP-1
• Reactivity: Rat
• Synonyms: HMGB-1 (High Mobility Group Protein B1); Rat HMGB-1 (High Mobility Group Protein B1) CLIA Kit; HMGB1; HMG1; HMG3; SBP-1; Amphoterin; HMGB-1 clia kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant HMGB-1. No significant cross-reactivity or interference between HMGB-1 and analogues was observed.
• Sequence Length: 215

• Assay Type: Sandwich
• Detection Range: 12.5-800pg/mL
• Sensitivity: Min: 7.5pg/mL; Max: 800pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HMGB-1 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of HMGB-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HMGB-1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HMGB-1. You can calculate the concentration of HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 926459557
• NCBI GeneID: 3146
• NCBI Accession #: NP_001300822.1
• NCBI GenBank Nucleotide #: NM_001313893.1
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1
• UniProt Entry Name: HMGB1_HUMAN

NCBI Description

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus; cytosol

Molecular Function: C-X-C chemokine binding; lipopolysaccharide binding; cytokine activity; lyase activity; bubble DNA binding; transcription factor binding; protein binding; RAGE receptor binding; four-way junction DNA binding; double-stranded DNA binding; damaged DNA binding; chromatin binding; transcription factor activity; DNA bending activity; single-stranded DNA binding; chemoattractant activity

Biological Process: positive regulation of mismatch repair; DNA topological change; apoptosis; positive regulation of apoptosis; programmed cell death; positive regulation of caspase activity; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; DNA geometric change; DNA recombination; elevation of cytosolic calcium ion concentration; regulation of tolerance induction; positive regulation of MAPKKK cascade; positive chemotaxis; dendritic cell chemotaxis; DNA ligation during DNA repair; DNA fragmentation during apoptosis; inflammatory response; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding; negative regulation of transcriptional preinitiation complex assembly; V(D)J recombination; regulation of restriction endodeoxyribonuclease activity; negative regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 secretion; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; innate immune response; positive regulation of transcription from RNA polymerase II promoter; regulation of autophagy; activation of innate immune response