Rat Glypican 4 (GPC4) ELISA Kit | GPC4 elisa kit

Rat Glypican 4 (GPC4) ELISA Kit

Cat. Num. AAA2024929

• Gene Names: GPC4; K-glypican
• Reactivity: Rat
• Synonyms: Glypican 4 (GPC4); Rat Glypican 4 (GPC4) ELISA Kit; K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4; GPC4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glypican 4 (GPC4).; No significant cross-reactivity or interference between Glypican 4 (GPC4) and analogues was observed.
• Sequence Length: 556

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 6.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glypican 4 (GPC4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glypican 4 (GPC4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPC4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glypican 4 (GPC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glypican 4 (GPC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glypican 4 (GPC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glypican 4 (GPC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GPC4 elisa kit

Tumor immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 21614525
• NCBI GeneID: 2239
• NCBI Accession #: NP_001439.2
• NCBI GenBank Nucleotide #: NM_001448.2
• Molecular Weight: 54,910 Da
• NCBI Official Full Name: glypican-4
• NCBI Official Synonym Full Names: glypican 4
• NCBI Official Symbol: GPC4
• NCBI Official Synonym Symbols: K-glypican
• NCBI Protein Information: glypican-4
• UniProt Protein Name: Glypican-4
• Protein Family: Glypican
• UniProt Gene Name: GPC4
• UniProt Entry Name: GPC4_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: proteinaceous extracellular matrix; extracellular space; lysosomal lumen; Golgi lumen; integral to plasma membrane; plasma membrane; nucleus; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; cell proliferation; phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1