Typical Testing Data/Standard Curve (for reference only)
Bovine Glypican 4 ELISA Kit | GPC4 elisa kit
Bovine Glypican 4 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GPC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC4 antibody and an GPC4-HRP conjugate. The assay sample and buffer are incubated together with GPC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC4 concentration since GPC4 from samples and GPC4-HRP conjugate compete for the anti-GPC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC4 from the sample, fewer sites are left to bind GPC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: Xq26.1
Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; extracellular space; Golgi lumen; integral to plasma membrane; plasma membrane; nucleus; external side of plasma membrane
Molecular Function: heparan sulfate proteoglycan binding
Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; phototransduction, visible light; cell proliferation; anatomical structure morphogenesis; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process
Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1
Research Articles on GPC4
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Product Notes
The Bovine GPC4 gpc4 (Catalog #AAA741876) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741876 ELISA Kit recognizes Bovine GPC4. It is sometimes possible for the material contained within the vial of "Glypican 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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