Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamine-dependent NAD (NADSYN1) Standard Curve.)
Rat Glutamine-Dependent NAD (NADSYN1) ELISA Kit | NADSYN1 elisa kit
Rat Glutamine-Dependent NAD (NADSYN1) ELISA Kit
Reactivity
Rat
Synonyms
Glutamine-Dependent NAD (NADSYN1); Rat Glutamine-Dependent NAD (NADSYN1) ELISA Kit; NADSYN1; FLJ10631; FLJ36703; FLJ40627; glutamine-dependent NAD synthetase; NADSYN1 elisa kit
Reactivity
Rat
Specificity
Rat Glutamine-dependent NAD (NADSYN1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NADSYN1. No significant cross-reactivity or interference between Rat NADSYN1 and analogues was observed.
Sequence Length
725
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamine-dependent NAD (NADSYN1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamine-dependent NAD (NADSYN1) Standard Curve.)
Related Product Information for NADSYN1 elisa kit
Description: This Rat Glutamine-dependent NAD (NADSYN1) ELISA Kit employs a two-site sandwich ELISA to quantitate NADSYN1 in samples. An antibody specific for NADSYN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NADSYN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NADSYN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NADSYN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD).
Background: Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glutamine-dependent NAD(+) synthetase
NCBI Official Synonym Full Names
NAD synthetase 1
NCBI Official Symbol
Nadsyn1
NCBI Protein Information
glutamine-dependent NAD(+) synthetase
UniProt Protein Name
Glutamine-dependent NAD(+) synthetase
Protein Family
UniProt Gene Name
Nadsyn1
UniProt Synonym Gene Names
Qns1
UniProt Entry Name
NADE_RAT
NCBI Description
human homolog catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD) [RGD, Feb 2006]
Similar Products
Product Notes
The Rat NADSYN1 nadsyn1 (Catalog #AAA9712175) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712175 ELISA Kit recognizes Rat NADSYN1. It is sometimes possible for the material contained within the vial of "Glutamine-Dependent NAD (NADSYN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
