Rat Glucose Transporter 3 (GLUT3) ELISA Kit | GLUT3 elisa kit

Rat Glucose Transporter 3 (GLUT3) ELISA Kit

Cat. Num. AAA450806

• Gene Names: Slc2a3; GLUT3
• Reactivity: Rat
• Synonyms: Glucose Transporter 3 (GLUT3); Rat Glucose Transporter 3 (GLUT3) ELISA Kit; SLC2A3; Solute Carrier Family 2(Facilitated Glucose Transporter)Member 3; Glucose transporter type 3; brain; GLUT3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of GLUT3. No significant cross-reactivity or interference between GLUT3 and analogues was observed.
• Sequence Length: 493

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.116ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLUT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLUT3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLUT3 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GLUT3 in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GLUT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GLUT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLUT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLUT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148747140
• NCBI GeneID: 25551
• NCBI Accession #: NP_058798.2
• NCBI GenBank Nucleotide #: NM_017102.2
• UniProt Accession #: Q07647; Q62729
• Molecular Weight: 53,581 Da
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 3
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 3
• NCBI Official Symbol: Slc2a3
• NCBI Official Synonym Symbols: GLUT3
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 3
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 3
• Protein Family: Glucose transporter
• UniProt Gene Name: Slc2a3
• UniProt Synonym Gene Names: Glut-3; Glut3; GLUT-3

NCBI Description

may act as a glucose transporter in neurons; may mediate increasd glucose uptake in response to neuronal injury [RGD, Feb 2006]

Uniprot Description

Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport.