Rat Glucose Transporter 3 (GLUT3) ELISA Kit | GLUT3 elisa kit

Rat Glucose Transporter 3 (GLUT3) ELISA Kit

Cat. Num. AAA2023892

• Gene Names: SLC2A3; GLUT3
• Reactivity: Rat
• Synonyms: Glucose Transporter 3 (GLUT3); Rat Glucose Transporter 3 (GLUT3) ELISA Kit; SLC2A3; Solute Carrier Family 2(Facilitated Glucose Transporter)Member 3; Glucose transporter type 3; brain; GLUT3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of GLUT3. No significant cross-reactivity or interference between GLUT3 and analogues was observed.
• Sequence Length: 496

• Samples: Rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLUT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLUT3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLUT3 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GLUT3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GLUT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLUT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLUT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GLUT3 elisa kit

Signal transduction; Metabolic pathway; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 8927560
• NCBI GeneID: 6515
• UniProt Accession #: P11169; Q6I9U2; Q9UG15; B2R606; D3DUU6
• Molecular Weight: 53,924 Da
• NCBI Official Full Name: glucose transporter 3
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 3
• NCBI Official Symbol: SLC2A3
• NCBI Official Synonym Symbols: GLUT3
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 3
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 3
• Protein Family: Glucose transporter
• UniProt Gene Name: SLC2A3
• UniProt Synonym Gene Names: GLUT3; GLUT-3
• UniProt Entry Name: GTR3_HUMAN

Uniprot Description

GLUT3: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. It???s surface expression is induced by insulin. It is restricted in humans to brain, testis (in the spermatozoa), and skeletal muscle, mostly in triads of slow-twitch fibres. Detected only in the brain of mouse and rat. IHC demonstrates its present in neurons, mainly at the plasma membrane, and to a lesser extent on intracellular vesicles distinct from synaptic vesicles. Present in the a-granules of human platelets, where it is translocated to the cell surface in response to thrombin stimulation. Its surface expression is increased by insulin or insulin growth-factor in cultured L6 muscle cells.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: glucose transmembrane transporter activity

Biological Process: vitamin metabolic process; hexose transport; carbohydrate metabolic process; L-ascorbic acid metabolic process; pathogenesis; glucose transport; transmembrane transport; water-soluble vitamin metabolic process