Rabbit anti-Human, Mouse MAF1 Polyclonal Antibody | anti-MAF1 antibody

MAF1, ID (MAF1, Repressor of RNA polymerase III transcription MAF1 homolog) (APC)

Cat. Num. AAA6317946

• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAF1; Polyclonal Antibody; ID (MAF1; Repressor of RNA polymerase III transcription MAF1 homolog) (APC); Repressor of RNA polymerase III transcription MAF1 homolog; anti-MAF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAF1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from the Central region of human MAF1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MAF1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAF1 antibody detected the MAF1 protein (arrow).)

Immunohistochemistry (IHC)

(MAF1 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAF1 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MAF1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAF1 antibody

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.

Product Categories/Family for anti-MAF1 antibody

Antibodies; Enzymes; Polymerase

References

Kantidakis, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11823-11828(2010). Shor, B., et al. J. Biol. Chem. 285(20):15380-15392(2010). Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Rollins, J., et al. Int. J. Biol. Sci. 3(5):292-302(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 110349740
• NCBI GeneID: 84232
• NCBI Accession #: NP_115648.2
• NCBI GenBank Nucleotide #: NM_032272.4
• UniProt Accession #: Q9H063; D3DWL4
• Molecular Weight: 28,771 Da
• NCBI Official Full Name: repressor of RNA polymerase III transcription MAF1 homolog
• NCBI Official Synonym Full Names: MAF1 homolog (S. cerevisiae)
• NCBI Official Symbol: MAF1
• NCBI Protein Information: repressor of RNA polymerase III transcription MAF1 homolog; homolog of yeast MAF1
• UniProt Protein Name: Repressor of RNA polymerase III transcription MAF1 homolog
• Protein Family: Maf-like protein
• UniProt Gene Name: MAF1
• UniProt Entry Name: MAF1_HUMAN

NCBI Description

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq, Jul 2008]

Uniprot Description

MAF1: Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA. Belongs to the MAF1 family.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; axon; dendrite; cytoplasm; plasma membrane; nucleolus; intracellular; cytosol; nucleus

Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase III promoter