Rat Gastrokine 1 (GKN1) ELISA Kit | GKN1 elisa kit

Rat Gastrokine 1 (GKN1) ELISA Kit

Cat. Num. AAA2019113

• Gene Names: GKN1; FOV; CA11; AMP18; BRICD1; foveolin
• Reactivity: Rat
• Synonyms: Gastrokine 1 (GKN1); Rat Gastrokine 1 (GKN1) ELISA Kit; CA11; AMP18; BRICD1; FOV; Foveolin; 18 kDa antrum mucosa protein; GKN1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Gastrokine 1 (GKN1).; No significant cross-reactivity or interference between Gastrokine 1 (GKN1) and analogues was observed.
• Sequence Length: 199

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.069ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastrokine 1 (GKN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastrokine 1 (GKN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GKN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gastrokine 1 (GKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gastrokine 1 (GKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gastrokine 1 (GKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gastrokine 1 (GKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GKN1 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 94538357
• NCBI GeneID: 56287
• NCBI Accession #: NP_062563.3
• NCBI GenBank Nucleotide #: NM_019617.3
• UniProt Accession #: Q9NS71; Q8IUA9
• Molecular Weight: 21,999 Da
• NCBI Official Full Name: gastrokine-1
• NCBI Official Synonym Full Names: gastrokine 1
• NCBI Official Symbol: GKN1
• NCBI Official Synonym Symbols: FOV; CA11; AMP18; BRICD1; foveolin
• NCBI Protein Information: gastrokine-1
• UniProt Protein Name: Gastrokine-1
• Protein Family: Gastrokine
• UniProt Gene Name: GKN1
• UniProt Synonym Gene Names: AMP18; CA11; AMP-18
• UniProt Entry Name: GKN1_HUMAN

NCBI Description

The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. [provided by RefSeq, Jul 2008]

Uniprot Description

GKN1: Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium. Belongs to the gastrokine family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular region; secretory granule

Molecular Function: growth factor activity

Biological Process: positive regulation of cell division; digestion; positive regulation of cell proliferation