Typical Testing Data/Standard Curve (for reference only)
Monkey Gastrokine 1 ELISA Kit | GKN1 elisa kit
Monkey Gastrokine 1 ELISA Kit
Gene Names
GKN1; FOV; CA11; AMP18; BRICD1; foveolin
Reactivity
Monkey
Synonyms
Gastrokine 1; Monkey Gastrokine 1 ELISA Kit; GKN1 elisa kit
Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GKN1 elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This GKN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GKN1. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY GKN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GKN1. Standards or samples are then added to the microtiter plate wells and GKN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GKN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GKN1 are added to each well to “sandwich” the GKN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GKN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GKN1 concentration in each sample is interpolated from this standard curve.
INTENDED USE This GKN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GKN1. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY GKN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GKN1. Standards or samples are then added to the microtiter plate wells and GKN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GKN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GKN1 are added to each well to “sandwich” the GKN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GKN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GKN1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GKN1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,999 Da
NCBI Official Full Name
gastrokine-1
NCBI Official Synonym Full Names
gastrokine 1
NCBI Official Symbol
GKN1
NCBI Official Synonym Symbols
FOV; CA11; AMP18; BRICD1; foveolin
NCBI Protein Information
gastrokine-1; AMP-18; BRICHOS domain containing 1; 18 kDa antrum mucosa protein
UniProt Protein Name
Gastrokine-1
Protein Family
UniProt Gene Name
GKN1
UniProt Synonym Gene Names
AMP18; CA11; UNQ489/PRO1005; AMP-18
UniProt Entry Name
GKN1_HUMAN
NCBI Description
The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. [provided by RefSeq, Jul 2008]
Uniprot Description
GKN1: Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium. Belongs to the gastrokine family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: extracellular region; secretory granule
Molecular Function: growth factor activity
Biological Process: positive regulation of cell division; digestion; positive regulation of cell proliferation
Research Articles on GKN1
Similar Products
Product Notes
The Monkey GKN1 gkn1 (Catalog #AAA737727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737727 ELISA Kit recognizes Monkey GKN1. It is sometimes possible for the material contained within the vial of "Gastrokine 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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