Monkey Gastrokine 1 ELISA Kit | GKN1 elisa kit

Monkey Gastrokine 1 ELISA Kit

Cat. Num. AAA737727

• Gene Names: GKN1; FOV; CA11; AMP18; BRICD1; foveolin
• Reactivity: Monkey
• Synonyms: Gastrokine 1; Monkey Gastrokine 1 ELISA Kit; GKN1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GKN1 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This GKN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GKN1. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY GKN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GKN1. Standards or samples are then added to the microtiter plate wells and GKN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GKN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GKN1 are added to each well to “sandwich” the GKN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GKN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GKN1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GKN1 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 94538357
• NCBI GeneID: 56287
• NCBI Accession #: NP_062563.3
• NCBI GenBank Nucleotide #: NM_019617.3
• Molecular Weight: 21,999 Da
• NCBI Official Full Name: gastrokine-1
• NCBI Official Synonym Full Names: gastrokine 1
• NCBI Official Symbol: GKN1
• NCBI Official Synonym Symbols: FOV; CA11; AMP18; BRICD1; foveolin
• NCBI Protein Information: gastrokine-1; AMP-18; BRICHOS domain containing 1; 18 kDa antrum mucosa protein
• UniProt Protein Name: Gastrokine-1
• Protein Family: Gastrokine
• UniProt Gene Name: GKN1
• UniProt Synonym Gene Names: AMP18; CA11; UNQ489/PRO1005; AMP-18
• UniProt Entry Name: GKN1_HUMAN

NCBI Description

The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. [provided by RefSeq, Jul 2008]

Uniprot Description

GKN1: Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium. Belongs to the gastrokine family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular region; secretory granule

Molecular Function: growth factor activity

Biological Process: positive regulation of cell division; digestion; positive regulation of cell proliferation