Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Gastric inhibitory polypeptide (GIP) Standard Curve.)
Rat Gastric Inhibitory Polypeptide (GIP) ELISA Kit | GIP elisa kit
Rat Gastric Inhibitory Polypeptide (GIP) ELISA Kit
Gene Names
Gip; Gludins; RATGLUDINS
Reactivity
Rat
Synonyms
Gastric Inhibitory Polypeptide (GIP); Rat Gastric Inhibitory Polypeptide (GIP) ELISA Kit; GIP; Glucose-dependent insulinotropic polypeptide; GIP elisa kit
Reactivity
Rat
Specificity
Rat Gastric inhibitory polypeptide (GIP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GIP. No significant cross-reactivity or interference between Rat GIP and analogues was observed.
Sequence Length
144
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Gastric inhibitory polypeptide (GIP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Gastric inhibitory polypeptide (GIP) Standard Curve.)
Related Product Information for GIP elisa kit
Description: This Rat Gastric inhibitory polypeptide (GIP) ELISA Kit employs a two-site sandwich ELISA to quantitate GIP in samples. An antibody specific for GIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Gastric inhibitory polypeptide (GIP), is a member of the secretin family of hormones.GIP is derived from a 153-amino acid proprotein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide. It is synthesized by K cells, which are found in the mucosa of the duodenum and the jejunum of the gastrointestinal tract.Like all endocrine hormones, it is transported by blood. It is now believed that the function of GIP is to induce insulin secretion, which is primarily stimulated by hyperosmolarity of glucose in the duodenum. After this discovery, some researchers prefer the new name of glucose-dependent insulinotropic peptide, while retaining the acronym "GIP." The amount of insulin secreted is greater when glucose is administered orally than intravenously.
Background: Gastric inhibitory polypeptide (GIP), is a member of the secretin family of hormones.GIP is derived from a 153-amino acid proprotein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide. It is synthesized by K cells, which are found in the mucosa of the duodenum and the jejunum of the gastrointestinal tract.Like all endocrine hormones, it is transported by blood. It is now believed that the function of GIP is to induce insulin secretion, which is primarily stimulated by hyperosmolarity of glucose in the duodenum. After this discovery, some researchers prefer the new name of glucose-dependent insulinotropic peptide, while retaining the acronym "GIP." The amount of insulin secreted is greater when glucose is administered orally than intravenously.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
gastric inhibitory polypeptide
NCBI Official Synonym Full Names
gastric inhibitory polypeptide
NCBI Official Symbol
Gip
NCBI Official Synonym Symbols
Gludins; RATGLUDINS
NCBI Protein Information
gastric inhibitory polypeptide
UniProt Protein Name
Gastric inhibitory polypeptide
Protein Family
UniProt Gene Name
Gip
UniProt Synonym Gene Names
GIP
NCBI Description
gastrointestinal regulatory peptide that stimulates insulin secretion from pancreatic beta cells in the presence of glucose [RGD, Feb 2006]
Uniprot Description
Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
Research Articles on GIP
Similar Products
Product Notes
The Rat GIP gip (Catalog #AAA9712519) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712519 ELISA Kit recognizes Rat GIP. It is sometimes possible for the material contained within the vial of "Gastric Inhibitory Polypeptide (GIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
