Rat GAL3 ELISA Kit | GAL3 elisa kit

Rat GAL3(Galectin 3)ELISA Kit

Cat. Num. AAA2511742

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Reactivity: Rat
• Synonyms: GAL3; Rat GAL3(Galectin 3)ELISA Kit; GAL3 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat GAL3. No significant cross-reactivity or interference between Rat GAL3 and analogues was observed.
• Sequence Length: 200

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GAL3 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAL3 elisa kit

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAL3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GAL3. You can calculate the concentration of GAL3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 294345475
• NCBI GeneID: 3958
• NCBI Accession #: NP_001170859.1
• NCBI GenBank Nucleotide #: NM_001177388.1
• UniProt Accession #: P17931; Q16005; Q6IBA7; Q96J47; B2RC38
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: galectin-3 isoform 2
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3; 35 kDa lectin; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; laminin-binding protein; lectin L-29
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN

NCBI Description

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Extracellular matrix; Cell surface; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: spliceosome; extracellular matrix; proteinaceous extracellular matrix; extracellular space; membrane; cytoplasm; mitochondrial inner membrane; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development