Rat Free Fatty Acid Receptor 1 (FFAR1) ELISA Kit | FFAR1 elisa kit

Rat Free Fatty Acid Receptor 1 (FFAR1) ELISA Kit

Cat. Num. AAA2704836

• Gene Names: FFAR1; FFA1R; GPR40; GPCR40
• Reactivity: Rat
• Synonyms: Free Fatty Acid Receptor 1 (FFAR1); Rat Free Fatty Acid Receptor 1 (FFAR1) ELISA Kit; GPR40; FFA1R; G Protein-Coupled Receptor 40; FFAR1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Free Fatty Acid Receptor 1 (FFAR1).; No significant cross-reactivity or interference between Free Fatty Acid Receptor 1 (FFAR1) and analogues was observed.
• Sequence Length: 300

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Free Fatty Acid Receptor 1 (FFAR1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Free Fatty Acid Receptor 1 (FFAR1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FFAR1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Free Fatty Acid Receptor 1 (FFAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Free Fatty Acid Receptor 1 (FFAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Free Fatty Acid Receptor 1 (FFAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Fatty Acid Receptor 1 (FFAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885327
• NCBI GeneID: 2864
• NCBI Accession #: NP_005294.1
• NCBI GenBank Nucleotide #: NM_005303.2
• UniProt Accession #: O14842; Q0VAS2; Q4VBL4
• Molecular Weight: 31,457 Da
• NCBI Official Full Name: free fatty acid receptor 1
• NCBI Official Synonym Full Names: free fatty acid receptor 1
• NCBI Official Symbol: FFAR1
• NCBI Official Synonym Symbols: FFA1R; GPR40; GPCR40
• NCBI Protein Information: free fatty acid receptor 1
• UniProt Protein Name: Free fatty acid receptor 1
• Protein Family: Free fatty acid receptor
• UniProt Gene Name: FFAR1
• UniProt Synonym Gene Names: GPR40

NCBI Description

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq, Apr 2009]

Uniprot Description

FFAR1: Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G- proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: bioactive lipid receptor activity; G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity; lipid binding

Biological Process: G-protein coupled receptor signaling pathway; glucose homeostasis; insulin secretion; positive regulation of calcium ion transport; positive regulation of GTPase activity; positive regulation of insulin secretion