Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tankyrase-1 (TNKS) Standard Curve.)
Human Tankyrase-1 (TNKS) ELISA Kit | TNKS elisa kit
Human Tankyrase-1 (TNKS) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tankyrase-1 (TNKS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tankyrase-1 (TNKS) Standard Curve.)
Background: Tankyrase 1, a human telomeric poly(ADP-ribose) polymerase, was originally identified through its interaction with TRF1, a negative regulator of telomere length. Tankyrase 1 ADP-ribosylates TRF1 in vitro, and its overexpression induces telomere elongation in human cancer cells. Surprisingly, dissection of this domain reveals multiple discrete and overlapping binding sites for TRF1 and TAB182. Five well conserved ankyrin repeat clusters in tankyrase 1. Although each of the five ankyrin repeat clusters independently binds to TRF1, only three of the five bind toTAB182. These findings suggest that tankyrase 1 may act as a scaffold for large molecular mass complexes made up of multiple binding proteins. Potential roles for tankyrase 1-mediated higher order complexes at telomeres and at other subcellular sites.
NCBI and Uniprot Product Information
Uniprot Description
TNKS: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.4.2.30; Transferase
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: Golgi membrane; spindle pole; Golgi apparatus; pericentriolar material; chromosome, telomeric region; nuclear membrane; nuclear chromosome, telomeric region; nuclear pore; cytosol; chromosome, pericentric region
Molecular Function: protein binding; zinc ion binding; NAD+ ADP-ribosyltransferase activity
Biological Process: mitosis; protein polyubiquitination; mRNA transport; Wnt receptor signaling pathway; peptidyl-threonine phosphorylation; spindle assembly; negative regulation of DNA binding; positive regulation of telomere maintenance via telomerase; peptidyl-serine phosphorylation; protein amino acid ADP-ribosylation; protein transport; mitotic spindle organization and biogenesis; cell division; positive regulation of transcription from RNA polymerase II promoter; regulation of telomere maintenance via telomerase
Research Articles on TNKS
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Product Notes
The Human TNKS tnks (Catalog #AAA9713863) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713863 ELISA Kit recognizes Human TNKS. It is sometimes possible for the material contained within the vial of "Tankyrase-1 (TNKS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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