Rat Fibromodulin (FMOD) ELISA Kit | FMOD elisa kit

Rat Fibromodulin (FMOD) ELISA Kit

Cat. Num. AAA2703364

• Gene Names: FMOD; FM; SLRR2E
• Reactivity: Rat
• Synonyms: Fibromodulin (FMOD); Rat Fibromodulin (FMOD) ELISA Kit; SLRR2E; FM; Collagen-binding 59 kDa protein; Keratan sulfate proteoglycan fibromodulin; KSPG fibromodulin; FMOD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibromodulin (FMOD).; No significant cross-reactivity or interference between Fibromodulin (FMOD) and analogues was observed.
• Sequence Length: 376

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.125ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibromodulin (FMOD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibromodulin (FMOD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FMOD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibromodulin (FMOD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibromodulin (FMOD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibromodulin (FMOD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibromodulin (FMOD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71040111
• NCBI GeneID: 2331
• NCBI Accession #: NP_002014.2
• NCBI GenBank Nucleotide #: NM_002023.4
• UniProt Accession #: Q06828; Q15331; Q8IV47
• Molecular Weight: 43,179 Da
• NCBI Official Full Name: fibromodulin
• NCBI Official Synonym Full Names: fibromodulin
• NCBI Official Symbol: FMOD
• NCBI Official Synonym Symbols: FM; SLRR2E
• NCBI Protein Information: fibromodulin
• UniProt Protein Name: Fibromodulin
• Protein Family: Fibromodulin
• UniProt Gene Name: FMOD
• UniProt Synonym Gene Names: FM; SLRR2E; FM; KSPG fibromodulin

NCBI Description

Fibromodulin belongs to the family of small interstitial proteoglycans. The encoded protein possesses a central region containing leucine-rich repeats with 4 keratan sulfate chains, flanked by terminal domains containing disulphide bonds. Owing to the interaction with type I and type II collagen fibrils and in vitro inhibition of fibrillogenesis, the encoded protein may play a role in the assembly of extracellular matrix. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. Sequence variations in this gene may be associated with the pathogenesis of high myopia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis ().