Rat Fibroblast Growth Factor 6 (FGF6) ELISA Kit | FGF6 elisa kit

Rat Fibroblast Growth Factor 6 (FGF6) ELISA Kit

Cat. Num. AAA2700841

• Gene Names: FGF6; HST2; HBGF-6
• Reactivity: Rat
• Synonyms: Fibroblast Growth Factor 6 (FGF6); Rat Fibroblast Growth Factor 6 (FGF6) ELISA Kit; HBGF6; HST2; HST2 Oncogene; Heparin Secretory-Transforming Protein 2; Heparin-Binding Growth Factor 6; FGF6 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 6 (FGF6).; No significant cross-reactivity or interference between Fibroblast Growth Factor 6 (FGF6) and analogues was observed.
• Sequence Length: 208

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 6 (FGF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 6 (FGF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 6 (FGF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 6 (FGF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15147343
• NCBI GeneID: 2251
• NCBI Accession #: NP_066276.2
• NCBI GenBank Nucleotide #: NM_020996.2
• UniProt Accession #: P10767; Q0VAE1
• Molecular Weight: 22,905 Da
• NCBI Official Full Name: fibroblast growth factor 6
• NCBI Official Synonym Full Names: fibroblast growth factor 6
• NCBI Official Symbol: FGF6
• NCBI Official Synonym Symbols: HST2; HBGF-6
• NCBI Protein Information: fibroblast growth factor 6
• UniProt Protein Name: Fibroblast growth factor 6
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF6
• UniProt Synonym Gene Names: HST2; HSTF2; FGF-6; HST-2; HSTF-2; HBGF-6

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene displayed oncogenic transforming activity when transfected into mammalian cells. The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis, and is required for normal muscle regeneration.