Rat Fibroblast Activation Protein Alpha (FAPa) ELISA Kit | FAPa elisa kit

Rat Fibroblast Activation Protein Alpha (FAPa) ELISA Kit

Cat. Num. AAA2021908

• Gene Names: FAP; FAPA; SIMP; DPPIV
• Reactivity: Rat
• Synonyms: Fibroblast Activation Protein Alpha (FAPa); Rat Fibroblast Activation Protein Alpha (FAPa) ELISA Kit; APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease; FAPa elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Activation Protein Alpha (FAPa).; No significant cross-reactivity or interference between Fibroblast Activation Protein Alpha (FAPa) and analogues was observed.
• Sequence Length: 239

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Activation Protein Alpha (FAPa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Activation Protein Alpha (FAPa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FAPa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Activation Protein Alpha (FAPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Activation Protein Alpha (FAPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Activation Protein Alpha (FAPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Activation Protein Alpha (FAPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FAPa elisa kit

Tumor immunity; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 119631759
• NCBI GeneID: 2191
• UniProt Accession #: Q12884; O00199; Q53TP5; Q86Z29; Q99998; Q9UID4
• Molecular Weight: 26,954 Da
• NCBI Official Full Name: fibroblast activation protein, alpha, isoform CRA_b
• NCBI Official Synonym Full Names: fibroblast activation protein, alpha
• NCBI Official Symbol: FAP
• NCBI Official Synonym Symbols: FAPA; SIMP; DPPIV
• NCBI Protein Information: prolyl endopeptidase FAP
• UniProt Protein Name: Prolyl endopeptidase FAP
• UniProt Gene Name: FAP
• UniProt Synonym Gene Names: APCE
• UniProt Entry Name: SEPR_HUMAN

NCBI Description

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; EC 3.4.21.26; EC 3.4.14.5

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: extracellular space; cell surface; focal adhesion; lamellipodium; apical part of cell; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein dimerization activity; integrin binding; peptidase activity; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; serine-type endopeptidase activity; metalloendopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; proteolysis; cell adhesion; regulation of fibrinolysis