Rat Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 ELISA Kit | EIF4EBP1 elisa kit

Rat Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 ELISA Kit

Cat. Num. AAA3809353

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
• Reactivity: Rat
• Synonyms: Eukaryotic Translation Initiation Factor 4E-Binding Protein 1; Rat Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 ELISA Kit; EIF4EBP1 elisa kit

• Reactivity: Rat
• Sequence Length: 118

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 250 pg/ml-4000 pg/ml
• Sensitivity: 10 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EIF4EBP1 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofEUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN 1 in the sample, This EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN 1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusEUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN 1 concentration. The concentration ofEUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758258
• NCBI GeneID: 1978
• NCBI Accession #: NP_004086
• NCBI GenBank Nucleotide #: NP_004086.1
• UniProt Accession #: Q13541
• NCBI Official Full Name: eukaryotic translation initiation factor 4E-binding protein 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• Protein Family: Eukaryotic translation initiation factor 4E-binding protein
• UniProt Gene Name: EIF4EBP1
• UniProt Synonym Gene Names: 4E-BP1; eIF4E-binding protein 1; PHAS-I
• UniProt Entry Name: 4EBP1_HUMAN

NCBI Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Uniprot Description

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; response to ethanol; cellular protein metabolic process; TOR signaling pathway; translation; insulin receptor signaling pathway; translational initiation; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development