Typical Testing Data/Standard Curve (for reference only)
Porcine Dopamine Receptor D4 ELISA Kit | DRD4 elisa kit
Porcine Dopamine Receptor D4 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DRD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DRD4 antibody and an DRD4-HRP conjugate. The assay sample and buffer are incubated together with DRD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD4 concentration since DRD4 from samples and DRD4-HRP conjugate compete for the anti-DRD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD4 from the sample, fewer sites are left to bind DRD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq, Jul 2008]
Uniprot Description
DRD4: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cell soma; membrane; integral to plasma membrane; dendritic spine; plasma membrane; terminal button; cell cortex; vesicle membrane
Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; protein binding; potassium channel regulator activity; dopamine receptor activity; dopamine binding; drug binding; SH3 domain binding
Biological Process: circadian rhythm; synaptic transmission, dopaminergic; fear response; behavioral fear response; positive regulation of dopamine uptake; activation of MAPK activity; short-term memory; adult locomotory behavior; regulation of neurotransmitter secretion; positive regulation of kinase activity; regulation of calcium-mediated signaling; behavioral response to ethanol; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; regulation of inhibitory postsynaptic membrane potential; negative regulation of cAMP biosynthetic process; behavioral response to cocaine; negative regulation of protein secretion; negative regulation of adenylate cyclase activity; response to amphetamine; regulation of dopamine metabolic process; social behavior; arachidonic acid secretion; regulation of circadian rhythm; cellular calcium ion homeostasis; olfactory learning; retina development in camera-type eye; response to steroid hormone stimulus; photoperiodism; dopamine receptor signaling pathway; positive regulation of sodium:hydrogen antiporter activity
Disease: Novelty Seeking Personality Trait; Attention Deficit-hyperactivity Disorder
Research Articles on DRD4
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Product Notes
The Porcine DRD4 drd4 (Catalog #AAA745974) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745974 ELISA Kit recognizes Porcine DRD4. It is sometimes possible for the material contained within the vial of "Dopamine Receptor D4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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