Rat DNA mismatch repair protein Msh2 ELISA Kit | MSH2 elisa kit
Rat DNA mismatch repair protein Msh2 (MSH2) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
NCBI and Uniprot Product Information
NCBI Description
mismatch repair protein; may have an important role in spermatogenesis [RGD, Feb 2006]
Uniprot Description
MSH2: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2- MSH3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases. Interacts with SMARCAD1. Ubiquitously expressed. Belongs to the DNA mismatch repair MutS family. Two isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Tumor suppressor
Chromosomal Location of Human Ortholog: 6q12
Cellular Component: membrane; MutSalpha complex; MutSbeta complex; nuclear chromosome, telomeric region; nucleus
Molecular Function: ADP binding; ATP binding; ATPase activity; centromeric DNA binding; damaged DNA binding; dinucleotide insertion or deletion binding; dinucleotide repeat insertion binding; DNA binding; DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; double-stranded DNA binding; enzyme binding; four-way junction DNA binding; guanine/thymine mispair binding; loop DNA binding; magnesium ion binding; mismatched DNA binding; MutLalpha complex binding; oxidized purine DNA binding; protein C-terminus binding; protein homodimerization activity; protein kinase binding; single base insertion or deletion binding; single guanine insertion binding; single thymine insertion binding; single-stranded DNA binding; Y-form DNA binding
Biological Process: B cell differentiation; B cell mediated immunity; cell cycle arrest; cellular response to DNA damage stimulus; determination of adult lifespan; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; double-strand break repair; germ cell development; in utero embryonic development; intra-S DNA damage checkpoint; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; isotype switching; maintenance of DNA repeat elements; male gonad development; meiotic gene conversion; mismatch repair; negative regulation of DNA recombination; negative regulation of neuron apoptosis; negative regulation of reciprocal meiotic recombination; oxidative phosphorylation; positive regulation of helicase activity; positive regulation of isotype switching to IgA isotypes; positive regulation of isotype switching to IgG isotypes; postreplication repair; response to amino acid; response to drug; response to organic cyclic compound; response to UV-B; response to X-ray; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments; somatic recombination of immunoglobulin genes during immune response; spermatogenesis
Research Articles on MSH2
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Product Notes
The Rat MSH2 msh2 (Catalog #AAA285748) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285748 ELISA Kit recognizes Rat MSH2. It is sometimes possible for the material contained within the vial of "DNA mismatch repair protein Msh2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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