Rat DMP1 (Dentin matrix acidic phosphoprotein 1) ELISA Kit | DMP1 elisa kit

Rat DMP1 (Dentin matrix acidic phosphoprotein 1) ELISA Kit

Cat. Num. AAA7612462

• Gene Names: Dmp1; DENTMAT
• Reactivity: Rat
• Synonyms: DMP1 (Dentin matrix acidic phosphoprotein 1); Rat DMP1 (Dentin matrix acidic phosphoprotein 1) ELISA Kit; Dentin matrix acidic phosphoprotein 1; DMP-1; Dentin matrix protein 1; AG1; Dmp1; DMP1; DMP1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of DMP1. No significant cross-reactivity or interference between DMP1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DMP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7673986
• NCBI GeneID: 25312
• UniProt Accession #: P98193
• Molecular Weight: 53,058 Da
• NCBI Official Full Name: Dentin matrix acidic phosphoprotein 1
• NCBI Official Synonym Full Names: dentin matrix acidic phosphoprotein 1
• NCBI Official Symbol: Dmp1
• NCBI Official Synonym Symbols: DENTMAT
• NCBI Protein Information: dentin matrix acidic phosphoprotein 1
• UniProt Protein Name: Dentin matrix acidic phosphoprotein 1
• Protein Family: Dentin matrix acidic phosphoprotein
• UniProt Gene Name: Dmp1
• UniProt Synonym Gene Names: DMP-1; Dentin matrix protein 1

NCBI Description

acidic protein associated with the dentin matrix; may be a matrix-associated acidic phosphoprotein; may have a regulatory role in dentin mineralization [RGD, Feb 2006]

Uniprot Description

DMP1: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. Defects in DMP1 are the cause of rickets hypophosphatemic autosomal recessive type 1 (ARHR1). A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14p22

Cellular Component: cytoplasm; nucleus; proteinaceous extracellular matrix

Molecular Function: extracellular matrix binding; Hsp70 protein binding

Biological Process: biomineral formation; extracellular matrix organization; ossification; regulation of bone mineralization