Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Death domain-associated protein 6 (DAXX) Standard Curve.)
Rat Death Domain-Associated Protein 6 (DAXX) ELISA Kit | DAXX elisa kit
Rat Death Domain-Associated Protein 6 (DAXX) ELISA Kit
Reactivity
Rat
Synonyms
Death Domain-Associated Protein 6 (DAXX); Rat Death Domain-Associated Protein 6 (DAXX) ELISA Kit; DAXX; DADB-159G18.9; BING2; DAP6; EAP1; MGC126245; MGC126246; CENP-C binding protein; ETS1-associated protein 1; Fas-binding protein; death-associated protein 6; DAXX elisa kit
Reactivity
Rat
Specificity
Rat Death domain-associated protein 6 (DAXX) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DAXX. No significant cross-reactivity or interference between Rat DAXX and analogues was observed.
Sequence Length
731
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Death domain-associated protein 6 (DAXX) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Death domain-associated protein 6 (DAXX) Standard Curve.)
Related Product Information for DAXX elisa kit
Description: This Rat Death domain-associated protein 6 (DAXX) ELISA Kit employs a two-site sandwich ELISA to quantitate DAXX in samples. An antibody specific for DAXX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAXX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAXX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAXX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Death domain-associated protein 6 (DAXX) encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
Background: Death domain-associated protein 6 (DAXX) encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Death domain-associated protein 6
NCBI Official Synonym Full Names
death-domain associated protein
NCBI Official Symbol
Daxx
NCBI Protein Information
death domain-associated protein 6
UniProt Protein Name
Death domain-associated protein 6
Protein Family
UniProt Gene Name
Daxx
UniProt Entry Name
DAXX_RAT
NCBI Description
mouse homolog acts as a transcriptional repressor and plays a role in apoptosis [RGD, Feb 2006]
Research Articles on DAXX
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Product Notes
The Rat DAXX daxx (Catalog #AAA9712467) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712467 ELISA Kit recognizes Rat DAXX. It is sometimes possible for the material contained within the vial of "Death Domain-Associated Protein 6 (DAXX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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