Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Death-associated protein kinase 3 (DAPK3) Standard Curve.)
Rat Death-Associated Protein Kinase 3 (DAPK3) ELISA Kit | DAPK3 elisa kit
Rat Death-Associated Protein Kinase 3 (DAPK3) ELISA Kit
Gene Names
Dapk3; Dapkl
Reactivity
Rat
Synonyms
Death-Associated Protein Kinase 3 (DAPK3); Rat Death-Associated Protein Kinase 3 (DAPK3) ELISA Kit; DAPK3; FLJ36473; ZIP; ZIPK; ZIP kinase isoform; DAPK3 elisa kit
Reactivity
Rat
Specificity
Rat Death-associated protein kinase 3 (DAPK3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DAPK3. No significant cross-reactivity or interference between Rat DAPK3 and analogues was observed.
Sequence Length
448
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Death-associated protein kinase 3 (DAPK3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Death-associated protein kinase 3 (DAPK3) Standard Curve.)
Related Product Information for DAPK3 elisa kit
Description: This Rat Death-associated protein kinase 3 (DAPK3) ELISA Kit employs a two-site sandwich ELISA to quantitate DAPK3 in samples. An antibody specific for DAPK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAPK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAPK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAPK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.
Background: Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
death-associated protein kinase 3
NCBI Official Synonym Full Names
death-associated protein kinase 3
NCBI Official Symbol
Dapk3
NCBI Official Synonym Symbols
Dapkl
NCBI Protein Information
death-associated protein kinase 3
UniProt Protein Name
Death-associated protein kinase 3
Protein Family
UniProt Gene Name
Dapk3
UniProt Synonym Gene Names
Zipk; DAP kinase 3; Dlk
UniProt Entry Name
DAPK3_RAT
NCBI Description
calmodulin (CaM)-regulated protein kinase; may be involved in apoptosis and neuronal development [RGD, Feb 2006]
Research Articles on DAPK3
Similar Products
Product Notes
The Rat DAPK3 dapk3 (Catalog #AAA9712470) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712470 ELISA Kit recognizes Rat DAPK3. It is sometimes possible for the material contained within the vial of "Death-Associated Protein Kinase 3 (DAPK3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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