Typical Testing Data/Standard Curve (for reference only)
Mouse Neurofilament, Light Polypeptide (NEFL) ELISA Kit | NEFL elisa kit
Mouse Neurofilament, Light Polypeptide (NEFL) High Sensitive ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to NEFL has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NEFL and unlabeled NEFL (Standards or samples) with the pre-coated antibody specific to NEFL. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NEFL in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NEFL in the sample.
NCBI and Uniprot Product Information
Uniprot Description
NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 14|14 D1
Cellular Component: axon; cytoplasm; growth cone; intermediate filament; myelin sheath; neurofilament; neuron projection; TSC1-TSC2 complex
Molecular Function: identical protein binding; phospholipase binding; protein binding; protein binding, bridging; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; structural constituent of cytoskeleton
Biological Process: anterograde axon cargo transport; axon regeneration in the peripheral nervous system; axon transport of mitochondrion; intermediate filament bundle assembly; intermediate filament organization; intermediate filament polymerization and/or depolymerization; locomotion; microtubule cytoskeleton organization and biogenesis; negative regulation of neuron apoptosis; neurite morphogenesis; neurofilament bundle assembly; neurofilament cytoskeleton organization and biogenesis; neuromuscular process controlling balance; positive regulation of axonogenesis; protein polymerization; regulation of axon diameter; retrograde axon cargo transport
Research Articles on NEFL
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Product Notes
The Mouse NEFL nefl (Catalog #AAA2087077) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2087077 ELISA Kit recognizes Mouse NEFL. It is sometimes possible for the material contained within the vial of "Neurofilament, Light Polypeptide (NEFL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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