Rat Cyclin Dependent Kinase 2 (CDK2) ELISA Kit | CDK2 elisa kit

Rat Cyclin Dependent Kinase 2 (CDK2) ELISA Kit

Cat. Num. AAA2023872

• Reactivity: Rat
• Synonyms: Cyclin Dependent Kinase 2 (CDK2); Rat Cyclin Dependent Kinase 2 (CDK2) ELISA Kit; CDKN2; p33(CDK2); Cell division protein kinase 2; p33 protein kinase; CDK2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 2 (CDK2).; No significant cross-reactivity or interference between Cyclin Dependent Kinase 2 (CDK2) and analogues was observed.
• Sequence Length: –

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.133ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase 2 (CDK2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase 2 (CDK2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDK2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 2 (CDK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 2 (CDK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 2 (CDK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 2 (CDK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CDK2 elisa kit

Enzyme & Kinase; Metabolic pathway; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 41054836
• NCBI GeneID: 362817
• NCBI Accession #: NP_955795.1
• NCBI GenBank Nucleotide #: NM_199501.1
• UniProt Accession #: Q63699; O09136
• Molecular Weight: – Da
• NCBI Official Full Name: cyclin-dependent kinase 2
• NCBI Official Synonym Full Names: cyclin dependent kinase 2
• NCBI Official Symbol: Cdk2
• NCBI Protein Information: cyclin-dependent kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk2
• UniProt Synonym Gene Names: Cdkn2
• UniProt Entry Name: CDK2_RAT

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Cell cycle regulation; EC 2.7.11.22; Protein kinase, CMGC; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Cellular Component: Y chromosome; Cajal body; transcription factor complex; chromosome, telomeric region; intracellular membrane-bound organelle; X chromosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; microtubule organizing center; condensed chromosome; nucleus; cytosol; endosome

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; metal ion binding; protein complex binding; histone kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: lens development in camera-type eye; response to drug; mitosis; organ regeneration; response to cAMP; regulation of cell cycle; response to toxin; histone phosphorylation; positive regulation of transcription, DNA-dependent; DNA repair; protein amino acid phosphorylation; response to estradiol stimulus; peptidyl-serine phosphorylation; response to ethanol; response to cadmium ion; meiotic cell cycle; cellular response to insulin stimulus; cell division; positive regulation of cell proliferation; Ras protein signal transduction; response to electrical stimulus; potassium ion transport; positive regulation of DNA replication initiation; G1/S transition of mitotic cell cycle