Rat Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1) ELISA Kit | HSD11B1 elisa kit
Rat Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1) ELISA Kit
Reactivity
Rat
Synonyms
Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1); Rat Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1) ELISA Kit; 11-DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; MGC13539; SDR26C1; 11-beta-hydroxysteroid dehydrogenase 1; OTTHUMP00000034650; corticosteroid 11-beta-dehydrogenase isozyme 1; short chain dehydrogenase; HSD11B1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat HSD11B1. No significant cross-reactivity or interference between Rat HSD11B1 and analogues was observed.
Asssay Type
Quantitive Sandwich
Sample Types
Serum, Plasma, Other biological fluids
Detection Wavelength
450nm
Sample Volume
1-200uL
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Test Principle
This assay employs a two-site sandwich ELISA to quantitate HSD11B1 in samples. An antibody specific for HSD11B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSD11B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSD11B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSD11B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Assay Duration
1-4.5h
Preparation and Storage
Store at 2-8 degree C
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
32,125 Da
NCBI Official Full Name
Corticosteroid 11-beta-dehydrogenase isozyme 1
UniProt Protein Name
Corticosteroid 11-beta-dehydrogenase isozyme 1
Protein Family
UniProt Gene Name
HSD11B1
UniProt Synonym Gene Names
11-DH; 11-beta-HSD1
UniProt Entry Name
DHI1_RABIT
Uniprot Description
Function: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone.
Catalytic activity: An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.1 PublicationManual assertion based on experiment in:Ref.1
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Product Notes
The Rat HSD11B1 hsd11b1 (Catalog #AAA2801755) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2801755 ELISA Kit recognizes Rat HSD11B1. It is sometimes possible for the material contained within the vial of "Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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