Typical Testing Data/Standard Curve (for reference only)
Rat Contactin-associated protein-like 2 (CNTNAP2) ELISA Kit | CNTNAP2 elisa kit
Rat Contactin-associated protein-like 2 (CNTNAP2) ELISA Kit
Gene Names
Cntnap2; Caspr2; mKIAA0868; 5430425M22Rik
Reactivity
Rat
Synonyms
Contactin-associated protein-like 2 (CNTNAP2); Rat Contactin-associated protein-like 2 (CNTNAP2) ELISA Kit; CNTNAP2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of CNTNAP2. No significant cross-reactivity or interference between CNTNAP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNTNAP2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CNTNAP2 elisa kit
Intended Uses: This CNTNAP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CNTNAP2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CNTNAP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CNTNAP2 antibody and an CNTNAP2-HRP conjugate. The assay sample and buffer are incubated together with CNTNAP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNTNAP2 concentration since CNTNAP2 from samples and CNTNAP2-HRP conjugate compete for the anti-CNTNAP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNTNAP2 from the sample, fewer sites are left to bind CNTNAP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNTNAP2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CNTNAP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CNTNAP2 antibody and an CNTNAP2-HRP conjugate. The assay sample and buffer are incubated together with CNTNAP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNTNAP2 concentration since CNTNAP2 from samples and CNTNAP2-HRP conjugate compete for the anti-CNTNAP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNTNAP2 from the sample, fewer sites are left to bind CNTNAP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNTNAP2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CNTNAP2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,899 Da
NCBI Official Full Name
contactin-associated protein-like 2 isoform a
NCBI Official Synonym Full Names
contactin associated protein-like 2
NCBI Official Symbol
Cntnap2
NCBI Official Synonym Symbols
Caspr2; mKIAA0868; 5430425M22Rik
NCBI Protein Information
contactin-associated protein-like 2; cell recognition molecule Caspr2
UniProt Protein Name
Contactin-associated protein-like 2
Protein Family
UniProt Gene Name
Cntnap2
UniProt Synonym Gene Names
Kiaa0868
UniProt Entry Name
CNTP2_MOUSE
Uniprot Description
CNTNAP2: May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. Associates with KCNA2. Predominantly expressed in nervous system. Belongs to the neurexin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: Golgi apparatus; voltage-gated potassium channel complex; cell projection; cell surface; membrane; early endosome; integral to membrane; axolemma
Molecular Function: protein binding; enzyme binding; protease binding
Biological Process: adult behavior; clustering of voltage-gated potassium channels; neuron recognition; social behavior; learning; cell adhesion; neurite development
Research Articles on CNTNAP2
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Product Notes
The Rat CNTNAP2 cntnap2 (Catalog #AAA7230045) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230045 ELISA Kit recognizes Rat CNTNAP2. It is sometimes possible for the material contained within the vial of "Contactin-associated protein-like 2 (CNTNAP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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