Rat Complement factor H-related protein 2 (CFHR2) ELISA Kit | CFHR2 elisa kit

Rat Complement factor H-related protein 2 (CFHR2) ELISA Kit

Cat. Num. AAA7239247

• Gene Names: CFHR2; FHR2; HFL3; CFHL2
• Reactivity: Rat
• Synonyms: Complement factor H-related protein 2 (CFHR2); Rat Complement factor H-related protein 2 (CFHR2) ELISA Kit; CFHR2 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This CFHR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CFHR2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFHR2 elisa kit

Principle of the assay: CFHR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFHR2 antibody and an CFHR2-HRP conjugate. The assay sample and buffer are incubated together with CFHR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFHR2 concentration since CFHR2 from samples and CFHR2-HRP conjugate compete for the anti-CFHR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CFHR2 from the sample, fewer sites are left to bind CFHR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFHR2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CFHR2 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 5031695
• NCBI GeneID: 3080
• NCBI Accession #: NP_005657.1
• NCBI GenBank Nucleotide #: NM_005666.2
• Molecular Weight: 27,897 Da
• NCBI Official Full Name: complement factor H-related protein 2
• NCBI Official Synonym Full Names: complement factor H-related 2
• NCBI Official Symbol: CFHR2
• NCBI Official Synonym Symbols: FHR2; HFL3; CFHL2
• NCBI Protein Information: complement factor H-related protein 2; FHR-2; DDESK59; h factor-like 3; factor H-related gene 2; h factor-like protein 2; H factor (complement)-like 3
• UniProt Protein Name: Complement factor H-related protein 2
• Protein Family: Complement factor H-related protein
• UniProt Gene Name: CFHR2
• UniProt Synonym Gene Names: CFHL2; FHR2; HFL3; FHR-2
• UniProt Entry Name: FHR2_HUMAN

NCBI Description

This gene belongs to a family of complement factor H-related genes (CFHR), which are clustered together with complement factor H gene on chromosome 1, and are involved in regulation of complement. Mutations in CFHR genes have been associated with dense deposit disease and atypical haemolytic-uraemic syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

CFHR2: Might be involved in complement regulation. Can associate with lipoproteins and may play a role in lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: extracellular region