Rat Complement 1 Inhibitor (C1INH) ELISA Kit | C1INH elisa kit

Rat Complement 1 Inhibitor (C1INH) ELISA Kit

Cat. Num. AAA2020533

• Reactivity: Rat
• Synonyms: Complement 1 Inhibitor (C1INH); Rat Complement 1 Inhibitor (C1INH) ELISA Kit; SERPING1; C1IN; C1-INH; C1NH; HAE1; HAE2; Serpin Peptidase Inhibitor Clade G Member 1; Angioedema Hereditary; C1 Inhibitor; C1 Esterase Inhibitor; C1INH elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement 1 Inhibitor (C1INH). ; No significant cross-reactivity or interference between Complement 1 Inhibitor (C1INH) and analogues was observed.

• Samples: Serum, plasma and other biological fluids
• Assay Type: Competitive
• Detection Range: 6.17-500ng/mL
• Sensitivity: Typically less than 2.26ng/mL.
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively. ; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. ; CV(%) = SD/meanX100 ; Intra-Assay: CV<10% ; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards;; 2. Add 50uL standard or sample to each well.; And then add 50uL prepared Detection Reagent A immediately.; Shake and mix. Incubate 1 hour at 37 degree C;; 3. Aspirate and wash 3 times;; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;; 5. Aspirate and wash 5 times;; 6. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
• Preparation and Storage: For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.; ; For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.; ; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C1INH elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Complement 1 Inhibitor (C1INH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Complement 1 Inhibitor (C1INH) and unlabeled Complement 1 Inhibitor (C1INH) (Standards or samples) with the pre-coated antibody specific to Complement 1 Inhibitor (C1INH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Complement 1 Inhibitor (C1INH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Complement 1 Inhibitor (C1INH) in the sample.