Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sphingolipid delta (DEGS2) Standard Curve.)
Human Sphingolipid delta (DEGS2) ELISA Kit | DEGS2 elisa kit
Human Sphingolipid delta (DEGS2) ELISA Kit
Gene Names
DEGS2; DES2; FADS8; C14orf66
Reactivity
Human
Synonyms
Sphingolipid delta (DEGS2); Human Sphingolipid delta (DEGS2) ELISA Kit; DEGS2; C14orf66; DES2; FADS8; degenerative spermatocyte homolog 2; lipid desaturase; sphingolipid C4-hydroxylase/delta 4-desaturase; sphingolipid delta 4 desaturase/C-4 hydroxylase; sphingolipid delta(4)-desa; DEGS2 elisa kit
Reactivity
Human
Specificity
Human Sphingolipid delta (DEGS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEGS2. No significant cross-reactivity or interference between Human DEGS2 and analogues was observed.
Sequence Length
323
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sphingolipid delta (DEGS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sphingolipid delta (DEGS2) Standard Curve.)
Related Product Information for DEGS2 elisa kit
Description: This Human Sphingolipid delta (DEGS2) ELISA Kit employs a two-site sandwich ELISA to quantitate DEGS2 in samples. An antibody specific for DEGS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEGS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEGS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEGS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DEGS2 encodes a bifunctional enzyme that is involved in the biosynthesis of phytosphingolipids in human skin and in other phytosphingolipid-containing tissues. This enzyme can act as a sphingolipid delta(4)-desaturase, and also as a sphingolipid C4-hydroxylase.
Background: DEGS2 encodes a bifunctional enzyme that is involved in the biosynthesis of phytosphingolipids in human skin and in other phytosphingolipid-containing tissues. This enzyme can act as a sphingolipid delta(4)-desaturase, and also as a sphingolipid C4-hydroxylase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sphingolipid delta(4)-desaturase/C4-monooxygenase DES2
NCBI Official Synonym Full Names
delta 4-desaturase, sphingolipid 2
NCBI Official Symbol
DEGS2
NCBI Official Synonym Symbols
DES2; FADS8; C14orf66
NCBI Protein Information
sphingolipid delta(4)-desaturase/C4-monooxygenase DES2
UniProt Protein Name
Sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
UniProt Gene Name
DEGS2
UniProt Synonym Gene Names
C14orf66
UniProt Entry Name
DEGS2_HUMAN
NCBI Description
This gene encodes a bifunctional enzyme that is involved in the biosynthesis of phytosphingolipids in human skin and in other phytosphingolipid-containing tissues. This enzyme can act as a sphingolipid delta(4)-desaturase, and also as a sphingolipid C4-hydroxylase. [provided by RefSeq, Oct 2008]
Uniprot Description
Function: Bifunctional enzyme which acts as both a sphingolipid delta(4)-desaturase and a sphingolipid C4-hydroxylase
By similarity. UniProtKB Q8R2F2
Pathway: Membrane lipid metabolism; sphingolipid biosynthesis.
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein
By similarity UniProtKB Q8R2F2.
Tissue specificity: Highly expressed in skin, intestine and kidney. Ref.1
Induction: Up-regulated during keratinocyte differentiation. Not expressed at day 0 or day 3 after differentiation, detected on day 6 and increases by day 9. Ref.1
Sequence similarities: Belongs to the fatty acid desaturase family. DEGS subfamily.
Research Articles on DEGS2
Similar Products
Product Notes
The Human DEGS2 degs2 (Catalog #AAA9715762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715762 ELISA Kit recognizes Human DEGS2. It is sometimes possible for the material contained within the vial of "Sphingolipid delta (DEGS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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