Rat Collagen Type II (COL2) ELISA Kit | COL2 elisa kit

Rat Collagen Type II (COL2) ELISA Kit

Cat. Num. AAA2702065

• Gene Names: COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
• Reactivity: Rat
• Synonyms: Collagen Type II (COL2); Rat Collagen Type II (COL2) ELISA Kit; COL2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL2. No significant cross-reactivity or interference between COL2 and analogues was observed.
• Sequence Length: 1487

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.29ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL2 in rat serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 95132445
• NCBI GeneID: 1280
• Molecular Weight: 29,781 Da
• NCBI Official Full Name: Collagen, type II, alpha 1
• NCBI Official Synonym Full Names: collagen type II alpha 1 chain
• NCBI Official Symbol: COL2A1
• NCBI Official Synonym Symbols: AOM; ANFH; SEDC; STL1; COL11A3
• NCBI Protein Information: collagen alpha-1(II) chain
• UniProt Protein Name: Collagen alpha-1(II) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL2A1

NCBI Description

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.