Typical Testing Data/Standard Curve (for reference only)
Rat Collagen triple helix repeat-containing protein 1 (CTHRC1) ELISA Kit | CTHRC1 elisa kit
Rat Collagen triple helix repeat-containing protein 1 (CTHRC1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CTHRC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CTHRC1 antibody and an CTHRC1-HRP conjugate. The assay sample and buffer are incubated together with CTHRC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTHRC1 concentration since CTHRC1 from samples and CTHRC1-HRP conjugate compete for the anti-CTHRC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTHRC1 from the sample, fewer sites are left to bind CTHRC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTHRC1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This locus encodes a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012]
Uniprot Description
CTHRC1: May act as a negative regulator of collagen matrix deposition. Defects in CTHRC1 may be a cause of Barrett esophagus (BE). A condition characterized by a metaplastic change in which normal esophageal squamous epithelium is replaced by a columnar and intestinal-type epithelium. Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes. Genetic variants in CTHRC1 have been found in individuals with Barrett esophagus and are thought to contribute to disease susceptibility. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 8q22.3
Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; cytoplasm
Molecular Function: Wnt-protein binding; frizzled binding
Biological Process: positive regulation of osteoblast differentiation; inner ear receptor stereocilium organization and biogenesis; cell migration; positive regulation of protein binding; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of osteoblast proliferation
Disease: Barrett Esophagus
Research Articles on CTHRC1
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Product Notes
The Rat CTHRC1 cthrc1 (Catalog #AAA7224636) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7224636 ELISA Kit recognizes Rat CTHRC1. It is sometimes possible for the material contained within the vial of "Collagen triple helix repeat-containing protein 1 (CTHRC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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